University of Colorado, Boulder

3 ARTICLES PUBLISHED IN JoVE

Medicine

Assessment of Vascular Function in Patients With Chronic Kidney Disease

Kristen L. Jablonski¹, Emily Decker¹, Loni Perrenoud¹, Jessica Kendrick¹, Michel Chonchol¹, Douglas R. Seals², Diana Jalal¹

¹Division of Renal Diseases and Hypertension, University of Colorado, Denver, ²Department of Integrative Physiology, University of Colorado, Boulder

The degree of vascular dysfunction and contributing physiological mechanisms can be assessed in patients with chronic kidney disease by measuring brachial artery flow-mediated dilation, aortic pulse-wave velocity, and vascular endothelial cell protein expression.

Biology

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Jerome E. Lee¹, Rui Yi^1,2

¹Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, ²Linda Crnic Institute for Down Syndrome, University of Colorado, Denver

MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.

Medicine

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Russell T. Burke¹, James D. Orth¹

¹Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder

Here, we describe a method of long-term time-lapse microscopy to longitudinally track single cells in response to anti-cancer therapeutics.