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University of Bologna

11 ARTICLES PUBLISHED IN JoVE

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Education

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Luca Mazzei 1, Stefano Ciurli 1, Barbara Zambelli 1
1Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology, University of Bologna

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

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Biology

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Alessandra Stefan 1,2, Alessandro Ceccarelli 1, Emanuele Conte 1, Alejandro Montón Silva 1, Alejandro Hochkoeppler 1,2
1Department of Pharmacy and Biotechnology, University of Bologna, 2CSGI, Department of Chemistry, University of Firenze

Protein co-expression is a powerful alternative to the reconstitution in vitro of protein complexes, and is of help in performing biochemical and genetic tests in vivo. Here we report on the use of protein co-expression in Escherichia coli to obtain protein complexes, and to tune the mutation frequency of cells.

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Bioengineering

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
Lucia Cavigli 1, Francesca Tatini 1, Claudia Borri 2, Fulvio Ratto 1, Sonia Centi 1, Alberto Cini 3, Beatrice Lelli 4, Paolo Matteini 1, Roberto Pini 1
1Institute of Applied Physics, Italian National Research Council, 2Department of Experimental and Clinical Biomedical Sciences, University of Florence, Firenze, 3Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, 4Department of Pharmacy and Biotechnology, University of Bologna

We show the preparation and address the feasibility of cellular vehicles containing gold nanorods for the photoacoustic imaging of cancer.

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Bioengineering

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
Manuela Ferracin 1, Irene Salamon 2, Laura Lupini 2, Elena Miotto 2, Silvia Sabbioni 3, Massimo Negrini 4
1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara

A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.

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Behavior

Ultrasound Cyclo Plasty in Eyes with Glaucoma
Giuseppe Giannaccare 1, Stefano Sebastiani 1, Emilio C. Campos 1
1Ophthalmology Unit, S.Orsola-Malpighi Teaching Hospital, DIMES, University of Bologna

Glaucoma is a chronic disease with progressive degeneration of optic nerve fibers resulting in decreased visual field. Elevated intraocular pressure is considered the most important and the only treatable risk factor. This manuscript describes a simple, surgeon-friendly, non-incisional technique, named Ultrasound Cyclo Plasty, for reducing intraocular pressure in glaucoma patients.

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Medicine

Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report
Paolo Giuseppe Limoli 1, Enzo Maria Vingolo 2, Celeste Limoli 1, Sergio Zaccaria Scalinci 3, Marcella Nebbioso 4
1Low Vision Research Centre of Milan, 2Department of Ophthalmology, A. Fiorini Hospital, Sapienza University of Rome, 3Glaucoma and Low Vision Study Center, Department of General Surgery and Organ Transplants, University of Bologna, 4Department of Sense Organs, Faculty of Medicine and Odontology, Sapienza University of Rome

The goal of this study is to assess whether the suprachoroidal graft of adipose-derived stem cells included in the stromal vascular fraction and platelets derived from platelet-rich plasma by the Limoli Retinal Restoration Technique can improve visual acuity and retinal sensitivity responses in eyes affected by dry age-related macular degeneration.

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Medicine

Intense Pulsed Light for the Treatment of Dry Eye Owing to Meibomian Gland Dysfunction
Luca Vigo 1, Giuseppe Giannaccare 2, Stefano Sebastiani 2, Marco Pellegrini 2, Francesco Carones 1
1Carones Ophthalmology Center, 2Ophthalmology Unit, S.Orsola-Malpighi University Hospital, DIMES, University of Bologna

Dry eye disease is an increasingly common condition, which strongly impair patients' life quality. Recently, a new device employing intense pulsed light, specifically designed for the periocular area, has been shown to improve tear film stability and ocular discomfort symptoms in dry eye disease owing to meibomian gland dysfunction.

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Neuroscience

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery
Matteo Zoli *1,2, Lia Talozzi *2, Micaela Mitolo 3, Raffaele Lodi 2,4, Diego Mazzatenta *1,2, Caterina Tonon *2,3
1IRCCS Istituto delle Scienze Neurologiche di Bologna, Center for the Diagnosis and Treatment of Hypothalamic-Pituitary Diseases - Pituitary Unit, 2Department of Biomedical and NeuroMotor Sciences, University of Bologna, 3IRCCS Istituto delle Scienze Neurologiche di Bologna, Functional and Molecular Neuroimaging Unit, 4IRCCS Istituto delle Scienze Neurologiche di Bologna

We present a protocol to integrate diffusion MRI tractography in patient work-up to endoscopic endonasal surgery for a skull base tumor. The methods for adopting these neuroimaging studies in the pre- and intra-operative phases are described.

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JoVE Core

Lung CT Segmentation to Identify Consolidations and Ground Glass Areas for Quantitative Assesment of SARS-CoV Pneumonia
Arrigo Cattabriga *1, Maria Adriana Cocozza *1, Giulio Vara *1, Francesca Coppola 1, Rita Golfieri 1
1Department of Diagnostic and Specialty Medicine, Policlinico Sant'Orsola-Malpighi, University of Bologna

The aim of this protocol is to provide a time efficient way to segment volumes of interest on high-resolution CT scans to use for further radiomics analysis.

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Neuroscience

High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures
Vito Antonio Baldassarro 1
1Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna

We describe the production of mixed cultures of astrocytes and oligodendrocyte precursor cells derived from fetal or adult neural stem cells differentiating into mature oligodendrocytes, and in vitro modeling of noxious stimuli. The coupling with a cell-based high-content screening technique builds a reliable and robust drug screening system.

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Bioengineering

An Ex Vivo Porcine Model for Hydrodynamic Testing of Experimental Aortic Valve Procedures and Novel Medical Devices
V. Reed LaSala 1, Halil Beqaj 2, Mingze Sun 1, Sabrina Castagnini 3, Senay Ustunel 1, Elizabeth Cordoves 2, Kavya Rajesh 2, Sophia Jackman 2, David Kalfa 1,4
1Department of Surgery, Columbia University Medical Center, 2College of Physicians and Surgeons, Columbia University, 3School of Medicine and Surgery, University of Bologna, 4Division of Cardiac, Thoracic, and Vascular Surgery, Section of Pediatric and Congenital Cardiac Surgery, New York-Presbyterian Morgan Stanley Children’s Hospital, Columbia University Medical Center

We present a method for mounting a porcine aortic valve on a pulse duplicator to test its hydrodynamic properties. This method can be used to determine the change in hydrodynamics after the application of an experimental procedure or novel medical device prior to use in a large animal model.

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