Beth Israel Deaconess Medical Center and Harvard Medical School

4 ARTICLES PUBLISHED IN JoVE

Biology

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Janine M. Preble¹, Christina A. Pacak², Hiroshi Kondo¹, Allison A. MacKay², Douglas B. Cowan², James D. McCully¹

¹Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, ²Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital and Department of Anesthesia, Harvard Medical School

A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.

Bioengineering

Rat Model of Adhesive Capsulitis of the Shoulder

Stephen M. Okajima^*1, M. Belen Cubria^*1, Sharri J. Mortensen¹, Juan C. Villa-Camacho¹, Philip Hanna¹, Aron Lechtig¹, Miguel Perez-Viloria¹, Patrick Williamson¹, Mark W. Grinstaff^*3, Edward K. Rodriguez^*2, Ara Nazarian^*1,4

¹Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, ²Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, ³Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, ⁴Department of Orthopaedic Surgery, Yerevan State Medical University

This protocol presents an in vivo rat model of adhesive capsulitis. The model includes an internal fixation of the glenohumeral joint with extra-articular suture fixation for an extended time, resulting in a decreased rotational range of motion (ROM) and increased joint stiffness.

Medicine

A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion

Ilias P. Doulamis¹, Alvise Guariento¹, Mossab Y. Saeed¹, Rio S. Nomoto¹, Thomas Duignan¹, Pedro J. del Nido¹, James D. McCully¹

¹Department of Cardiac Surgery, Boston Children’s Hospital, Harvard Medical School

This study presents a highly reproducible large animal model of renal ischemia-reperfusion injury in swine using temporary percutaneous bilateral balloon-catheter occlusion of the renal arteries for 60 min and reperfusion for 24 h.

Biology

Extended 78% Hepatectomy in a Mouse Surgical Model

Phillip Brennan¹, Nyah Patel¹, Tarek Aridi¹, Michelle Zhan¹, Cleide Angolano¹, Christiane Ferran^1,2

¹Division of Vascular and Endovascular Surgery and the Center for Vascular Biology Research, Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, ²The Transplant Institute and the Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School

The mouse model of partial 2/3 (66%) hepatectomy is well described in the literature, but more extended hepatectomies mimicking small-for-size syndrome after liver transplantation have seldom been used. We describe an extended 78% hepatectomy procedure in a mouse model that results in approximately 50% postoperative lethality in healthy mice.