JoVE Logo

Sign In

Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

1 ARTICLES PUBLISHED IN JoVE

image

JoVE Journal

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
Jeffrey Kroon *1, Anna E. Daniel *1, Mark Hoogenboezem 1, Jaap D. van Buul 1
1Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Leukocytes cross the endothelial monolayer using the paracellular or the transcellular route. We developed a simple assay to follow the distribution of endogenous junctional VE-cadherin and PECAM-1 during leukocyte transendothelial migration under physiological flow to discriminate between the two transmigration routes.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved