Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological FlowJeffrey Kroon *1, Anna E. Daniel *1, Mark Hoogenboezem 1, Jaap D. van Buul 1
1Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam
Leukocytes cross the endothelial monolayer using the paracellular or the transcellular route. We developed a simple assay to follow the distribution of endogenous junctional VE-cadherin and PECAM-1 during leukocyte transendothelial migration under physiological flow to discriminate between the two transmigration routes.