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University Hospital Münster

9 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice
Stefan Bittner 1,2, Ali M. Afzali 1, Heinz Wiendl 1, Sven G. Meuth 1,3
1Department of Neurology, University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF), Münster, 3Institute of Physiology – Neuropathophysiology, University of Münster

Experimental autoimmune encephalomyelitis (EAE) is an established animal model of multiple sclerosis. C57BL/6 mice are immunized with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 (MOG35-55), resulting in an ascending flaccid paralysis caused by autoreactive immune cells in the central nervous system. Protocols for disease induction and monitoring will be discussed.

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Medicine

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
Markus Brückner *1, Philipp Lenz *1, Tobias M. Nowacki 1, Friederike Pott 1, Dirk Foell 2, Dominik Bettenworth 1
1Department of Medicine B, University Hospital Münster, 2Department of Pediatric Rheumatology and Immunology, University Children's Hospital Münster

Small animal imaging techniques allow serial diagnostic examinations and therapeutic interventions in vivo. Recently, the scope of applications has significantly widened and currently includes assessment of colonic tumor development, wound healing and monitoring of inflammation. This protocol illustrates these diverse potential applications of murine endoscopy.

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Neuroscience

Isolation of Primary Murine Brain Microvascular Endothelial Cells
Tobias Ruck 1, Stefan Bittner 1,2, Lisa Epping 1, Alexander M. Herrmann 1, Sven G. Meuth 1,3
1Department of Neurology, University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I — Neuropathophysiology I, University of Münster

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

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Immunology and Infection

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices
Kerstin Göbel 1, Stefan Bittner 1,2, Manuela Cerina 3, Alexander M. Herrmann 1, Heinz Wiendl 1, Sven G. Meuth 1,3
1Department of Neurology, University of Münster, 2Germany and Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I - Neuropathophysiology I, University of Münster

To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8+ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.

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Medicine

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
Philipp Lenz 1,2, Markus Brückner 1, Steffi Ketelhut 3, Jan Heidemann 4, Björn Kemper *3, Dominik Bettenworth *1
1Department of Medicine B, University Hospital Münster, 2Institute of Palliative Care, University Hospital Münster, 3Biomedical Technology Center, University of Münster, 4Department of Gastroenterology, Klinikum Bielefeld

Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.

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Immunology and Infection

An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction
Ivan Kuzmanov 1, Alexander M. Herrmann 1, Hans-Joachim Galla 2, Sven G. Meuth 1, Heinz Wiendl *1, Luisa Klotz *1
1Department of Neurology, University Hospital Münster, 2Institute of Biochemistry, University of Münster

Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

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Immunology and Infection

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
Andreas Schulte-Mecklenbeck 1, Urvashi Bhatia 1, Tilman Schneider-Hohendorf 1, Nicholas Schwab 1, Heinz Wiendl *1, Catharina C. Gross *1
1Department of Neurology with Institute of Translational Neurology, University Hospital Münster

Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.

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Medicine

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
Tobias M. Nowacki 1, Dominik Bettenworth 1, Markus Brückner 1, Friederike Cordes 1, Frank Lenze 1, Anne Becker 2, Moritz Wildgruber 2, Michel Eisenblätter 2
1Department of Medicine B, University Hospital Münster, 2Translational Research Imaging Center, Department of Clinical Radiology, University Hospital Münster

Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography.

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Neuroscience

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
Thomas Müntefering *1, Alexander P.E. Michels *1, Steffen Pfeuffer 1, Sven G. Meuth *1, Tobias Ruck *1
1Institute for Translational Neurology and Neurology Clinic, University of Muenster

Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".

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