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The Technion-Israel Institute of Technology

1 ARTICLES PUBLISHED IN JoVE

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Biology

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Nurit Livnat Levanon 1, Elena Vigonsky 1, Oded Lewinson 1
1Department of Biochemistry, The Bruce and Ruth Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology

Surface Plasmon Resonance (SPR) is a label-free method for detecting bio-molecular interactions in real time. Herein, a protocol for a membrane protein:receptor interaction experiment is described, while discussing the pros and cons of the technique.

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