Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)Nurit Livnat Levanon 1, Elena Vigonsky 1, Oded Lewinson 1
1Department of Biochemistry, The Bruce and Ruth Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology
Surface Plasmon Resonance (SPR) is a label-free method for detecting bio-molecular interactions in real time. Herein, a protocol for a membrane protein:receptor interaction experiment is described, while discussing the pros and cons of the technique.