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Sorbonne Universités

6 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
Philip O'Herron 1, Zhiming Shen 1, Zhongyang Lu 1, Adrien E. Schramm 1, Manuel Levy 1, Prakash Kara 1
1Department of Neuroscience, Medical University of South Carolina

A method is described for labeling neurons with fluorescent dyes in predetermined functional micro-domains of the neocortex. First, intrinsic signal optical imaging is used to obtain a functional map. Then two-photon microscopy is used to label and image neurons within a micro-domain of the map.

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Developmental Biology

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
Estelle Hirsinger 1, João Emanuel Carvalho 2, Christine Chevalier 1,3, Georges Lutfalla 4, Jean-François Nicolas 1, Nadine Peyriéras 5, Michael Schubert 2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

We report here the robust and efficient expression of fluorescent proteins after mRNA injection into unfertilized oocytes of Branchiostoma lanceolatum. The development of the microinjection technique in this basal chordate will pave the way for far-reaching technical innovations in this emerging model system, including in vivo imaging and gene-specific manipulations.

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Neuroscience

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
Tristan Altwegg-Boussac 1,2,3,4, Séverine Mahon 1,2,3,4, Mario Chavez 1,2,3,4, Stéphane Charpier 1,2,3,4, Adrien E. Schramm 1,2,3,4
1Inserm U1127, 2CNRS UMR 7225, 3UPMC Univ Paris 06, UMR S 1127, Sorbonne Universités, 4Institut du Cerveau et de la Moelle épinière (ICM)

This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.

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Biology

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
Anne-Sophie Perrin-Terrin 1,2, Florine Jeton 1,3, Aurelien Pichon 1,3,4, Alain Frugière 2, Jean-Paul Richalet 1,3, Laurence Bodineau 2, Nicolas Voituron 1,3
1Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363, University Paris 13, 2UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique, Sorbonne Universités, 3Laboratory of Excellence GR-Ex, 4Laboratory MOVE (EA 6314), University of Poitiers

Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.

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Neuroscience

Protocol for Isolating the Mouse Circle of Willis
Justine Claire Hur 1, Régis Blaise 1, Isabelle Limon 1
1UPMC Univ Paris 06, Sorbonne Universités

We describe here a reproducible protocol for isolating the mouse circle of Willis.

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Genetics

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
Sébastien Dussaud 1,2, Corinne Pardanaud-Glavieux 1, Claire Sauty-Colace 1, Philippe Ravassard 1
1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM, Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition, Sorbonne Universités

Here, we present a protocol to promote transgene integration and production of founder transgenic mice with high efficacy by a simple injection of a lentiviral vector in the perivitelline space of a fertilized oocyte.

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