High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin PolymerizationJana Ninković 1,3, Sabita Roy 1,2
1Department of Pharmacology, University of Minnesota, 2Department of Surgery, University of Minnesota, 33M Corporate Research Laboratory
Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.
