Monash University (Parkville Campus)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

Natalie L. Trevaskis¹, Luojuan Hu¹, Suzanne M. Caliph¹, Sifei Han¹, Christopher J.H. Porter¹

¹Drug Delivery, Disposition, and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus)

Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic molecules.