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U.S. Environmental Protection Agency

8 ARTICLES PUBLISHED IN JoVE

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Environment

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
G. Shay Fout 1, Jennifer L. Cashdollar 1, Eunice A. Varughese 1, Sandhya U. Parshionikar 2, Ann C. Grimm 1
1National Exposure Research Laboratory, U.S Environmental Protection Agency, 2Technical Support Center, Office of Ground Water and Drinking Water, U.S Environmental Protection Agency

EPA Method 1615 uses an electropositive filter to concentrate enteroviruses and noroviruses in environmental and drinking waters. This manuscript describes the procedure for collecting samples for Method 1615 analyses.

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Environment

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
G. Shay Fout 1, Jennifer L. Cashdollar 1
1National Exposure Research Laboratory, U.S. Environmental Protection Agency

Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

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Environment

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
G. Shay Fout 1, Jennifer L. Cashdollar 1, Shannon M. Griffin 1, Nichole E. Brinkman 1, Eunice A. Varughese 1, Sandhya U. Parshionikar 2
1National Exposure Research Laboratory, U.S. Environmental Protection Agency, 2Technical Services Center, Office of Ground Water and Drinking Water, U.S. Environmental Protection Agency

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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Immunology and Infection

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
Swinburne A. J. Augustine 1, Tarsha N. Eason 2, Kaneatra J. Simmons 1, Clarissa L. Curioso 3, Shannon M. Griffin 1, Malini K. D. Ramudit 1, Trevor R. Plunkett 4
1National Exposure Research Laboratory, U.S. Environmental Protection Agency, 2National Risk Management Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education, 4Department of Biological Sciences, McMicken College of Arts and Sciences, University of Cincinnati

In the current climate of scarce resources, new technologies are emerging that allow researchers to conduct studies cheaper, faster and with more precision. Here we describe the development of a bead-based salivary antibody multiplex immunoassay to measure human exposure to multiple environmental pathogens simultaneously.

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Biology

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Gail M Nelson 1, Jenna M Guynn 2, Brian N Chorley 1
1National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.

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Environment

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
James McCord 1, Mark Strynar 2
1Oak Ridge Institute for Science and Education, National Exposure Research Laboratory, U.S. Environmental Protection Agency, 2National Exposure Research Laboratory, U.S. Environmental Protection Agency

Here, we present a protocol for the sequential targeted quantification and non-targeted analysis of fluorinated compounds in water by mass spectrometry. This methodology provides quantitative levels of known fluorochemical compounds and identifies unknown chemicals in related samples with semi-quantitative estimates of their abundance.

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Genetics

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
Sara M. F. Vliet 1, Monique Hazemi 2, Donovan Blatz 2, Marissa Jensen 3, Sally Mayasich 4, Thomas R. Transue 5, Cody Simmons 5, Audrey Wilkinson 5, Carlie A. LaLone 6
1Office of Research and Development, Center for Computational Toxicology and Exposure, Scientific Computing and Data Curation Division, U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education, 3Swenson College of Science and Engineering, Department of Biology, University of Minnesota Duluth, 4University of Wisconsin-Madison Aquatic Sciences Center, 5General Dynamics Information Technology, Research Triangle Park, 6Office of Research and Development, Center for Computational Toxicology and Exposure, Great Lakes Toxicology and Ecology Division, U.S. Environmental Protection Agency

Here, we present a protocol to utilize the latest version of the US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool. This protocol demonstrates the application of the online tool to rapidly analyze protein conservation and provide customizable and easily interpretable predictions of chemical susceptibility across species.

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Engineering

Determining Viral Disinfection Efficacy of Hot Water Laundering
Anne Mikelonis *1, John Archer *1, Barbara Wyrzykowska-Ceradini 2, Eric Morris 3, Jonathan Sawyer 2, Timothy Chamberlain 2, Ahmed Abdel-Hady 2, Mariela Monge 4, Abderrahmane Touati 2
1Office of Research and Development, Center for Environmental Solutions and Emergency Response, Homeland Security and Materials Management Division, U.S. Environmental Protection Agency, 2Jacobs Technology Inc., 3Science Systems and Applications Inc., 4CSS Inc.

In response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, a laboratory protocol was developed to test the viral disinfection efficacy of hot water laundering of cloth face coverings, cotton scrubs, and denim pants. The Phi6 virus (bacteriophage) was used as the organism to test disinfection efficacy.

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