A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal OrganoidsJooske F. Van Lidth de Jeude 1, Jacqueline L. M. Vermeulen 1, Paula S. Montenegro-Miranda 1, Gijs R. Van den Brink 1, Jarom Heijmans 1
1Tytgat Institute for Liver and Intestinal Research, Department of Gasteroenterology and Hepatology, Academical Medical Center
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
