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National Tsing Hua University

11 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
Chihchen Chen 1, Bo-Ren Lin 1, Min-Yen Hsu 2, Chao-Min Cheng 1
1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Taichung Veterans General Hospital

This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.

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Bioengineering

Fabricating Cotton Analytical Devices
Shang-Chi Lin *1, Min-Yen Hsu *2, Chen-Meng Kuan 1, Fan-Gang Tseng 3, Chao-Min Cheng 4
1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Department of Ophthalmology, Taichung Veterans General Hospital, 3Department of Engineering and System Science, National Tsing Hua University, 4Institute of Biomedical Engineering, National Tsing Hua University

To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.

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JoVE Core

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
Tsung-Ting Shih *1, I-Hsiang Hsu *2, Ping-Hung Chen 1, Shun-Niang Chen 1, Sheng-Hao Tseng 1, Ming-Jay Deng 3, Yang-Wei Lin 4, Yuh-Chang Sun 1
1Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 2Center for Measurement Standards, Industrial Technology Research Institute, 3National Synchrotron Radiation Research Center, 4Department of Chemistry, National Changhua University of Education

The fabrication protocol of a dipole-assisted solid phase extraction microchip for the trace metal analysis is presented.

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Bioengineering

Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel
Hsin-Yi Hsieh 1, Chiao-Wen Chu 2, Ming-Hsuan Chiu 1, Shueh-Yao Chu 2, Tsu-Wei Huang 2, Fan-Gang Tseng 1,2
1Institute of NanoEngineering and MicroSystems, National Tsing Hua University, 2Department of Engineering and System, National Tsing Hua University

This article introduces a simple approach to providing non-continuous gradient static strains on a concentric cell-laden hydrogel to regulate cell alignment for tissue engineering.

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Immunology and Infection

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Ting-Yu Kuo 1, Zhi-Wei Hong 1, Chung-Che Tsai 1, Yu-Chi Yang 1, Hua-Wen Fu 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

A high yield method for one-step negative purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP) overexpressed in Escherichia coli by using diethylaminoethyl resins in batch mode is described. HP-NAP purified by this method is beneficial for the development of vaccines, drugs, or diagnostics for H. pylori-associated diseases.

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Bioengineering

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
Ching-Hui Lin 1,2, Hao-Chen Chang 1,2, Chia-Hsien Hsu 1,2,3
1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

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Genetics

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
Po C. Liu 1, Yi T. Lee 1, Chun Y. Wang 1, Ya-Tang Yang 1
1Electrical Engineering, National Tsing Hua University

We describe a low cost, configurable morbidostat that enables the characterization of antibiotic drug resistance by dynamically adjusting the drug concentration. The device can be integrated with a multiplexed microfluidic platform. The approach can be scaled up for laboratory antibiotic drug resistance studies.

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Engineering

Blue-hazard-free Candlelight OLED
Jwo-Huei Jou 1, Meenu Singh 1, Yu-Ting Su 1, Shih-Hao Liu 1, Zhe-Kai He 1
1Department of Materials Science and Engineering, National Tsing Hua University

We present a protocol for the fabrication of a blue-hazard-free candlelight organic light emitting diode (OLED) for eye protection and melatonin secretion.

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Bioengineering

Trapping of Micro Particles in Nanoplasmonic Optical Lattice
Dinesh Bhalothia 1, Ya-Tang Yang 1
1Electrical Engineering, National Tsing Hua University

We describe a procedure to optically trap micro-particles in nanoplasmonic optical lattice.

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Bioengineering

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
Cheng-Kun He 1,2, Ya-Wen Chen 3, Ssu-Han Wang 3, Chia-Hsien Hsu 1,2
1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, 2Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3National Institute of Cancer Research, National Health Research Institutes

This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.

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Engineering

A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education
Yu-Hao Guo 1, Chih-Hsien Lee 1, Ya-Tang Yang 1
1Electrical Engineering, National Tsing Hua University

We describe an educational kit that allows users to execute multiple experiments and gain hands-on experience on digital microfluidics.

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