School of Dentistry and Dental Research Institute, Seoul National University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Yun Sik Choi¹, Yong Cheol Kim¹, Keum Jin Baek¹, Youngnim Choi¹

¹Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University

Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.