Westmead Millennium Institute for Medical Research and University of Sydney

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

¹Centre for Cancer Research, Westmead Millennium Institute for Medical Research and University of Sydney

This protocol describes the use of bromodeoxyuridine (BrdU) uptake to permit the temporal tracking of cells that were in S phase at a specific point in time. Addition of DNA dyes and antibody labeling facilitates detailed analysis of the fate of the S phase cells at later times.