The Hospital for Sick Children, Toronto

2 ARTICLES PUBLISHED IN JoVE

Medicine

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

J. Moritz Kaths^1,2, Vinzent N. Spetzler¹, Nicolas Goldaracena¹, Juan Echeverri¹, Kristine S. Louis¹, Daniel B. Foltys³, Mari Strempel⁴, Paul Yip⁵, Rohan John⁵, Istvan Mucsi¹, Anand Ghanekar¹, Darius Bagli^6,7, Lisa Robinson², Markus Selzner¹

¹Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, ²Division of Nephrology, The Hospital for Sick Children, Toronto, ³Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, ⁴Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, ⁵Laboratory Medicine & Pathobiology, Toronto General Hospital, ⁶Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, ⁷Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.

Medicine

Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol

J. Moritz Kaths^1,2, Juan Echeverri^1,3, Nicolas Goldaracena¹, Kristine S. Louis¹, Paul Yip⁴, Rohan John⁴, Istvan Mucsi⁵, Anand Ghanekar¹, Darius Bagli⁶, Markus Selzner¹, Lisa A. Robinson²

¹Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, ²Division of Nephrology, The Hospital for Sick Children, ³Programa de Doctorat en Medicina, La Universitat Autónoma de Barcelona, ⁴Laboratory Medicine and Pathobiology, Toronto General Hospital, ⁵Department of Medicine, Toronto General Hospital, ⁶Departments of Surgery (Urology) & Physiology, Developmental & Stem Cell Biology, The Hospital for Sick Children

Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.