JoVE Logo

Sign In

West Virginia University School of Medicine

10 ARTICLES PUBLISHED IN JoVE

image

Medicine

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
Amanda Gatesman Ammer 1, Karen E. Hayes 1, Karen H. Martin 1, Lingqing Zhang 2, George A. Spirou 3, Scott A. Weed 1
1Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center, West Virginia University, 2Sensory Neuroscience Research Center, West Virginia University , 3Departments of Otolaryngology and Physiology, Center for Neuroscience, West Virginia University

A comprehensive overview of the techniques involved in generating a mouse model of oral cancer and quantitative monitoring of tumor invasion within the tongue through multi-photon microscopy of labeled cells is presented. This system can serve as a useful platform for the molecular assessment and drug efficacy of anti-invasive compounds.

image

Biology

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
Karen H. Martin 1, Karen E. Hayes 1, Elyse L. Walk 1, Amanda Gatesman Ammer 1, Steven M. Markwell 1, Scott A. Weed 1
1Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center, West Virginia University

We describe the prototypical method for producing microscope coverslips coated with fluorescent gelatin for visualizing invadopodia-mediated matrix degradation. Computational techniques using available software are presented for quantifying the resultant levels of matrix proteolysis by single cells within a mixed population and for multicellular groups encompassing entire microscopic fields.

image

Behavior

Asymmetric Walkway: A Novel Behavioral Assay for Studying Asymmetric Locomotion
Kiril Tuntevski 1, Ryan Ellison 1, Sergiy Yakovenko 1
1Neural Engineering Laboratory, Biomedical Research Center, West Virginia University School of Medicine

Here, we present a protocol to quantify precise stepping in rodents. Cortical and the spinal central pattern generator signals are required for precise foot-placement during obstructed locomotion. We report here the novel constrained walking task that directly examines precise stepping behavior.

image

Medicine

Modeling Chemotherapy Resistant Leukemia In Vitro
William L. Slone *1, Blake S. Moses *1, Rebecca Evans 1, Debbie Piktel 1, Karen H. Martin 1,2, William Petros 1, Michael Craig 1, Laura F. Gibson 1,3
1Alexander B. Osborn Hematopoietic Malignancy and Transplantation Program of the Mary Babb Randolph Cancer Center, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, 2Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, 3Department of Microbiology, Immunology and Cell Biology, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine

The current report summarizes a protocol that can be utilized to model the influence of the bone marrow microenvironment niche on leukemic cells with emphasis placed on enrichment of the most chemoresistant subpopulation.

image

Medicine

Establishing a Multidisciplinary Cavernous Carotid Injury Simulation to Train Neurosurgical, Otolaryngology, and Anesthesia Residents
Brandon Lucke-Wold 1, Haley E. Gillham 2, Mark Baskerville 2, William E. Cameron 2, Dawn Dillman 2, Caleb A. Haley 2, Michele Noles 2, Donn Spight 2, Jeremy N. Ciporen 2
1West Virginia University School of Medicine, 2Oregon Health & Science University

This protocol establishes a multidisciplinary model to train learners on management of cavernous carotid artery injury. Cadaveric heads undergo expanded endonasal approach and injury to the cavernous carotid artery, and perfusion pump simulates blood flow to the injury point. Learners are tasked with medical and surgical management over 3 scenarios.

image

Cancer Research

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
Andrey A. Bobko 1,2, Timothy D. Eubank 1,3, Benoit Driesschaert 1,2, Valery V. Khramtsov 1,2
1In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, 2Department of Biochemistry, West Virginia University School of Medicine, 3Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine

Low-field (L-band, 1.2 GHz) electron paramagnetic resonance using soluble nitroxyl and trityl probes is demonstrated for assessment of physiologically important parameters in the tumor microenvironment in mouse models of breast cancer.

image

Medicine

Development of a Cadaveric Multiport Model of Posterior Circulation Aneurysm Clipping for Neurosurgery and Otolaryngology Residents
Haley E. Gillham 1, Brandon Lucke-Wold 2, Aclan Dogan 1, Justin Cetas 1, William E. Cameron 1, Jeremy N. Ciporen 1
1Oregon Health & Science University, 2West Virginia University School of Medicine

A model protocol to train neurosurgery and otolaryngology resident learners on endoscopic transclival clipping of posterior circulation aneurysms is described. Two endoscopic approaches to access the silicone-injected or perfused posterior circulation of cadaveric heads are established for training. Learners are tasked with clipping of posterior circulation based on clinical scenarios.

image

Immunology and Infection

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
Brittany G. Seman *1, Jessica M. Povroznik *1,2, Jordan K. Vance 1, Travis W. Rawson 1, Cory M. Robinson 1,2
1Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, 2Vaccine Development Center at West Virginia University Health Sciences Center

Infection of neonatal mice with bioluminescent E. coli O1:K1:H7 results in a septic infection with significant pulmonary inflammation and lung pathology. Here, we describe procedures to model and further study neonatal sepsis using longitudinal intravital imaging in parallel with enumeration of systemic bacterial burdens, inflammatory profiling, and lung histopathology.

image

Measuring Cerebral Vasospasm after Subarachnoid Hemorrhage in Mice
William S. Dodd 1, Brandon Lucke-Wold 1, Koji Hosaka 1, Brian L. Hoh 1
1Department of Neurosurgery, College of Medicine, University of Florida

The present protocol provides a detailed procedure for inducing subarachnoid hemorrhage in mice via autologous blood injection to the anterior circulation and measuring delayed cerebral vasospasm by vascular gel casting.

image

Immunology and Infection

Neonatal Mouse Bone Marrow Isolation and Preparation of Bone Marrow-Derived Macrophages
Madhavi Annamanedi 1, Jordan K. Vance 1, Cory M. Robinson 1,2
1Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, 2Vaccine Development Center, West Virginia University Health Sciences Center

This protocol describes a non-enzymatic and straightforward method for isolating 7-9-day-old neonatal mouse bone marrow cells and generating differentiated macrophages using a supernatant of L929 cells as a source of granulocyte colony-stimulating factor (M-CSF). The bone marrow-derived macrophages were further analyzed for surface antigens F4/80, CD206, CD11b, and functional competency.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved