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A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

DOI :

10.3791/61609-v

August 12th, 2020

August 12th, 2020

5,953 Views

1Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, 2Vaccine Development Center at West Virginia University Health Sciences Center

Infection of neonatal mice with bioluminescent E. coli O1:K1:H7 results in a septic infection with significant pulmonary inflammation and lung pathology. Here, we describe procedures to model and further study neonatal sepsis using longitudinal intravital imaging in parallel with enumeration of systemic bacterial burdens, inflammatory profiling, and lung histopathology.

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