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Technion Israel Institute of Technology

4 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Engineered Vascularized Muscle Flap
Dana Egozi *1, Yulia Shandalov *2, Alina Freiman 2,3, Dekel Rosenfeld 2, David Ben-Shimol 4, Shulamit Levenberg 2
1Department of Plastic Surgery, Kaplan Medical Center, 2Biomedical Engineering, Technion Israel Institute of Technology, 3Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, 4Medicine Department, Technion Israel Institute of Technology

To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.

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JoVE Journal

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
Despina Soteriou 1, Lana Kostic 1, Egor Sedov 1, Yahav Yosefzon 1, Hermann Steller 2, Yaron Fuchs 1
1Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology, 2Strang Laboratory of Apoptosis and Cancer Biology, Howard Hughes Medical Institute, The Rockefeller University

An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

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Bioengineering

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
Ariel A. Szklanny 1, Dylan B. Neale 2, Joerg Lahann 2, Shulamit Levenberg 1
1Faculty of Biomedical Engineering, Technion, 2Department of Chemical Engineering and Biointerfaces Institute, University of Michigan, Ann Arbor

Engineered tissues heavily rely on proper vascular networks to provide vital nutrients and gases and remove metabolic waste. In this work, a stepwise seeding protocol of endothelial cells and support cells creates highly organized vascular networks in a high-throughput platform for studying developing vessel behavior in a controlled 3D environment.

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Bioengineering

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
Majd Machour 1, Ariel A. Szklanny 1, Shulamit Levenberg 1
1Faculty of Biomedical Engineering, Technion-Israel Institute of Technology

Engineered flaps require an incorporated functional vascular network. In this protocol, we present a method of fabricating a 3D printed tissue flap containing a hierarchical vascular network and its direct microsurgical anastomoses to rat femoral artery.

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