Technion Israel Institute of Technology

4 ARTICLES PUBLISHED IN JoVE

Bioengineering

Dana Egozi^*1, Yulia Shandalov^*2, Alina Freiman^2,3, Dekel Rosenfeld², David Ben-Shimol⁴, Shulamit Levenberg²

¹Department of Plastic Surgery, Kaplan Medical Center, ²Biomedical Engineering, Technion Israel Institute of Technology, ³Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, ⁴Medicine Department, Technion Israel Institute of Technology

To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.

JoVE Journal

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Despina Soteriou¹, Lana Kostic¹, Egor Sedov¹, Yahav Yosefzon¹, Hermann Steller², Yaron Fuchs¹

¹Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine, Technion Israel Institute of Technology, ²Strang Laboratory of Apoptosis and Cancer Biology, Howard Hughes Medical Institute, The Rockefeller University

An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.

Bioengineering

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Ariel A. Szklanny¹, Dylan B. Neale², Joerg Lahann², Shulamit Levenberg¹

¹Faculty of Biomedical Engineering, Technion, ²Department of Chemical Engineering and Biointerfaces Institute, University of Michigan, Ann Arbor

Engineered tissues heavily rely on proper vascular networks to provide vital nutrients and gases and remove metabolic waste. In this work, a stepwise seeding protocol of endothelial cells and support cells creates highly organized vascular networks in a high-throughput platform for studying developing vessel behavior in a controlled 3D environment.

Bioengineering

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Majd Machour¹, Ariel A. Szklanny¹, Shulamit Levenberg¹

¹Faculty of Biomedical Engineering, Technion-Israel Institute of Technology

Engineered flaps require an incorporated functional vascular network. In this protocol, we present a method of fabricating a 3D printed tissue flap containing a hierarchical vascular network and its direct microsurgical anastomoses to rat femoral artery.