Seoul National University Hospital

8 ARTICLES PUBLISHED IN JoVE

Neuroscience

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Sung Wook Park^1,2, Jin Hyoung Kim², Woo Jin Park³, Jeong Hun Kim^1,2,4

¹Department of Biomedical Sciences, Seoul National University College of Medicine, ²FARB Laboratory, Biomedical Research Institute, Seoul National University Hospital, ³College of Life Sciences, Gwangju Institute of Science and Technology, ⁴Department of Ophthalmology, Seoul National University College of Medicine

Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.

Medicine

Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs

Seong Joon Ahn^1,2,3, Hye Kyoung Hong¹, Young Mi Na¹, Sang Jun Park^1,2, Jeeyun Ahn^1,2,4, Jaeseong Oh⁵, Jae Yong Chung⁶, Kyu Hyung Park^1,2, Se Joon Woo^1,2

¹Department of Ophthalmology, Seoul National University Bundang Hospital, ²Department of Ophthalmology, College of Medicine, Seoul National University, ³Department of Ophthalmology, Hanyang University Hospital, ⁴Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, ⁵Department of Clinical Pharmacology, Seoul National University Hospital, ⁶Department of Clinical Pharmacology, Seoul National University Bundang Hospital

Rabbits are widely used to study the pharmacokinetics of intraocular drugs. We describe a method for conducting pharmacokinetic studies of intraocular drugs using rabbit eyes.

JoVE Core

Enhancement of the Initial Growth Rate of Agricultural Plants by Using Static Magnetic Fields

Seung C. Kim¹, Alex Mason², Wooseok Im³

¹College of Medicine, Yonsei University, ²Simon Fraser University, ³Biomedical Research Institute, Seoul National University Hospital

The goal of this protocol is to demonstrate the acceleration of the initial growth rate of plants by applying static magnetic fields with no external energy.

Behavior

Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain

Jaewon Beom^1,2, Jung Chan Lee^2,3,4, Jin Chul Paeng⁵, Tai Ryoon Han⁶, Moon Suk Bang⁷, Byung-Mo Oh⁷

¹Department of Rehabilitation Medicine, Chungnam National University Hospital, Daejeon, ²Department of Biomedical Engineering, Seoul National University College of Medicine, ³Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, ⁴Department of Biomedical Engineering, Seoul National University Hospital, ⁵Department of Nuclear Medicine, Seoul National University Hospital, Seoul National University College of Medicine, ⁶Department of Rehabilitation Medicine, Gangwon Do Rehabilitation Hospital, ⁷Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul National University College of Medicine

We applied repetitive transcranial magnetic stimulation (rTMS) to the unilateral hemisphere of rat brain, by placing a 25-mm figure-8 coil 1 cm lateral to the vertex on the biauricular line and angulating the coil by 45°. An in-house water cooling system was used for rTMS for more than 20 min.

Bioengineering

Robotic Mirror Therapy System for Functional Recovery of Hemiplegic Arms

Jaewon Beom^*1,2, Sukgyu Koh^*3, Hyung Seok Nam¹, Wonshik Kim³, Yoonjae Kim³, Han Gil Seo⁴, Byung-Mo Oh^4,5, Sun Gun Chung^4,5, Sungwan Kim^1,6

¹Department of Biomedical Engineering, Seoul National University College of Medicine, ²Department of Rehabilitation Medicine, Chungnam National University Hospital, ³Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, ⁴Department of Rehabilitation Medicine, Seoul National University Hospital, ⁵Seoul National University College of Medicine, ⁶Institute of Medical and Biological Engineering, Seoul National University

We developed a real-time mirror robot system for functional recovery of hemiplegic arms using automatic control technology, conducted a clinical study on healthy subjects, and determined tasks through feedback from rehabilitation doctors. This simple mirror robot can be applied effectively to occupational therapy in stroke patients with a hemiplegic arm.

Medicine

Surgical Labyrinthectomy of the Rat to Study the Vestibular System

Mun Young Chang¹, Moo Kyun Park², So Hyeon Park², Myung-Whan Suh², Jun Ho Lee², Seung Ha Oh²

¹Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, ²Department of Otolaryngology-Head and Neck Surgery, Seoul National University Hospital

This protocol describes the surgical labyrinthectomy of a rat, which is a useful method for studying the vestibular system.

Bioengineering

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Yeongjae Choi^*1, Hansol Choi^*1, Amos C. Lee^*2, Sunghoon Kwon^1,2,3,4

¹Department of Electrical and Computer Engineering, Seoul National University, ²Interdisciplinary Program for Bioengineering, Seoul National University, ³Institute of Entrepreneurial Bio Convergence, Seoul National University, ⁴Seoul National University Hospital Biomedical Research Institute, Seoul National University Hospital

We describe the detailed protocol for design, simulation, wet-lab experiments, and analysis for a reconfigurable DNA accordion rack of 6 by 6 meshes.

Bioengineering

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Daehan Kim¹, Gi-Hun Lee¹, Jiheum Park², Jung Chan Lee², Joong Yull Park¹

¹Department of Mechanical Engineering, Graduate School, Chung-Ang University, ²Department of Biomedical Engineering, College of Medicine, Seoul National University

We present a motor-powered centrifugal microfluidic device that can cultivate cell spheroids. Using this device, spheroids of single or multiple cell types could be easily cocultured under high gravity conditions.