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National Taiwan Ocean University

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
Hsien-San Hou 1, Hui-Fang Chang 1, Ji-Yen Cheng 1,2,3,4,5
1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang-Ming University, 3Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 4Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 5Ph.D. Program in Microbial Genomics, National Chung Hsing University

Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.

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Bioengineering

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli
Tzu-Yuan Chou 1, Yung-Shin Sun 2, Hsien-San Hou 3, Shang-Ying Wu 1, Yun Zhu 1, Ji-Yen Cheng 3, Kai-Yin Lo 1
1Department of Agricultural Chemistry, National Taiwan University, 2Department of Physics, Fu-Jen Catholic University, 3Research Center for Applied Sciences, Academia Sinica

Micro-fabricated devices integrated with fluidic components provide an in vitro platform for cell studies mimicking the in vivo micro-environment. We developed polymethylmethacrylate-based microfluidic chips for studying cellular responses under single or coexisting chemical/electrical/shear stress stimuli.

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Engineering

A High Performance Impedance-based Platform for Evaporation Rate Detection
Wei-Lung Chou 1, Pee-Yew Lee 2, Cheng-You Chen 2, Yu-Hsin Lin 3, Yung-Sheng Lin 4
1Department of Safety, Health and Environmental Engineering, Hungkuang University, 2Institute of Materials Engineering, National Taiwan Ocean University, 3Instrument Technology Research Center, National Applied Research Laboratories, 4Department of Chemical Engineering, National United University

This paper presents an impedance-based apparatus for evaporation rate detection of solutions. It offers clear advantages over a conventional weight loss approach: a fast response, high-sensitivity detection, a small sample requirement, multiple sample measurements, and easy disassembly for cleaning and reuse purposes.

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Chemistry

Synthesizing Sodium Tungstate and Sodium Molybdate Microcapsules via Bacterial Mineral Excretion
Pao-Hung Lin 1, Ying-Tang Huang 2, Fu-Wen Lin 3
1Department of Electronic and Computer Engineering, National Taiwan University of Science and Technology, 2Department of Marine Biotechnology, National Kaohsiung Marine University, 3Institute of Applied English, National Taiwan Ocean University

This work presents a protocol for manufacture sodium tungstate and sodium molybdate microcapsules via bacteria and their corresponding nanoparticles.

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Bioengineering

Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices
Hui-Fang Chang 1, Shih-En Chou 1, Ji-Yen Cheng 1,2,3,4
1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang Ming Chao Tung University, 3Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 4College of Engineering, Chang Gung University

In this study, we present a protocol for the differentiation of neural stem and progenitor cells (NPCs) solely induced by direct current (DC) pulse stimulation in a microfluidic system.

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