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Universidad Complutense de Madrid

8 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis
Juan Pellico 1,2, Jesús Ruiz-Cabello 1,2, Fernando Herranz 1
1Advanced Imaging Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III and CIBER de Enfermedades Respiratorias, 2Universidad Complutense de Madrid

Microwave technology enables extremely fast synthesis of iron oxide nanoparticles for atherosclerosis plaque characterization. The use of an aminobisphosphonate in the external side of the nanoparticle provides a fast accumulation in the atherosclerotic area.

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Biology

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
Silvia Murillo-Cuesta *1,2,3, Néstor Vallecillo *1, Rafael Cediel 1,2,4, Adelaida M. Celaya 1,2, Luis Lassaletta 3,5, Isabel Varela-Nieto 1,2,3, Julio Contreras 1,2,4
1Instituto de Investigaciones Biomédicas (IIBm) Alberto Sols CSIC-UAM, 2Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), 3Instituto de Investigación Sanitaria La Paz (IdiPAZ), 4Facultad de Veterinaria, Universidad Complutense de Madrid, 5Departmento de Otorrino laringología, Hospital Universitario La Paz

Two microsurgery approaches for local drug delivery to the inner ear are described here and compared in terms of impact on hearing parameters, cochlear cytoarchitecture and expression of inflammatory markers.

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Developmental Biology

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
María Jesús Sánchez-Calabuig 1,2, Francisco Alberto García-Vázquez 3, Ricardo Laguna-Barraza 1, Carlos Barros-García 4, Daniel García-Parraga 5, Dimitrios Rizos 1, Alfonso Gutiérrez Adan 1, José Félix Pérez-Gutíerrez 2
1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5Veterinary Services, L'Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

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Engineering

Growth and Electrostatic/chemical Properties of Metal/LaAlO3/SrTiO3 Heterostructures
Diogo Castro Vaz 1, Edouard Lesne 1,2, Anke Sander 1, Hiroshi Naganuma 1,3, Eric Jacquet 1, Jacobo Santamaria 1,4, Agnès Barthélémy 1, Manuel Bibes 1
1Unité Mixte de Physique CNRS/Thales, Université Paris-Saclay, 2Max Planck Institut für Mikrostrukturphysik, 3Department of Applied Physics, Tohoku University, 4Instituto de Magnetismo Aplicado, Universidad Complutense de Madrid

We fabricate metal/LaAlO3/SrTiO3 heterostructures using a combination of pulsed laser deposition and in situ magnetron sputtering. Through magnetotransport and in situ X-ray photoelectron spectroscopy experiments, we investigate the interplay between electrostatic and chemical phenomena of the quasi two-dimensional electron gas formed in this system.

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JoVE Journal

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
Jose Antonio Escudero 1,2, R Craig MacLean 3, Alvaro San Millan 4
1Department of Animal Health, Universidad Complutense de Madrid, 2Visavet Health Surveillance Centre, Universidad Complutense de Madrid, 3Department of Zoology, University of Oxford, 4Department of Microbiology, Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

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Environment

Laboratory and Field Protocol for Estimating Sheet Erosion Rates from Dendrogeomorphology
Jose Maria Bodoque 1, Juan Antonio Ballesteros-Cánovas 2,3, Juan Manuel Rubiales 4,5, Markus Stoffel 2,3
1University of Castilla-La Mancha (UCLM), 2Department of Earth Sciences, University of Geneva, 3Institute for Environmental Sciences, University of Geneva, 4Departamento de Sistemas y Recursos Naturales, Universidad Politécnica de Madrid, 5Departamento de Biodiversidad, Ecología y Evolución, Universidad Complutense de Madrid

Characterizing erosion from dendrogeomorphology has usually focused on accurately finding the starting time of root exposure, by examining macroscopic or cell level changes caused by exposure. Here, we offer a detailed description of different novel techniques to obtain more precise erosion rates from highly accurate microtopographic data.

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Biology

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /(T1)Magnetic Resonance Imaging
Irene Fernández-Barahona 1, Jesús Ruiz-Cabello 2, Fernando Herranz 1, Juan Pellico 3
1Nanobiotechnology, Molecular Imaging and Metabolomics Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2CIC biomaGUNE and CIBER de Enfermedades Respiratorias (CIBERES). Ikerbasque, Basque Foundation for Science, Universidad Complutense de Madrid (UCM), 3Advanced Imaging Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), CIBER de Enfermedades Respiratorias (CIBERES)

Here, we present a protocol to obtain 68Ga core-doped iron oxide nanoparticles via fast microwave-driven synthesis. The methodology renders PET/(T1)MRI nanoparticles with radiolabeling efficiencies higher than 90% and radiochemical purity of 99% in a 20-min synthesis.

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Medicine

Combining Augmented Reality and 3D Printing to Display Patient Models on a Smartphone
Rafael Moreta-Martinez 1,2, David García-Mato 1,2, Mónica García-Sevilla 1,2, Rubén Pérez-Mañanes 2,3,4, José A. Calvo-Haro 2,3,4, Javier Pascau 1,2
1Department of Bioengineering and Aerospace Engineering, Universidad Carlos III de Madrid, 2Instituto de Investigación Sanitaria Gregorio Marañón, 3Department of Orthopedic Surgery and Traumatology, Hospital General Universitario Gregorio Marañón, 4Department of Surgery, Faculty of Medicine, Universidad Complutense de Madrid

Presented here is a method to design an augmented reality smartphone application for the visualization of anatomical three-dimensional models of patients using a 3D-printed reference marker.

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