A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in WatersYiping Cao 1, Meredith R. Raith 1, John F. Griffith 1
1Department of Microbiology, Southern California Costal Water Research Project Authority
This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.