Southern California Costal Water Research Project Authority

1 ARTICLES PUBLISHED IN JoVE

Environment

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Yiping Cao¹, Meredith R. Raith¹, John F. Griffith¹

¹Department of Microbiology, Southern California Costal Water Research Project Authority

This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.