Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical ResearchAlexander E. Stover 1, Siranush Herculian 1, Maria G. Banuelos 1, Samantha L. Navarro 1, Michael P. Jenkins 1, Philip H. Schwartz 1
1National Human Neural Stem Cell Resource, Childrens Hospital of Orange County Research Institute
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
