JoVE Logo

Sign In

Johns Hopkins University, School of Medicine

4 ARTICLES PUBLISHED IN JoVE

image

Medicine

Adapted Resistance Training Improves Strength in Eight Weeks in Individuals with Multiple Sclerosis
Jennifer L. Keller 1, Nora Fritz 1,2, Chen Chun Chiang 1, Allen Jiang 1, Tziporah Thompson 3, Nicole Cornet 1, Scott D. Newsome 4, Peter A. Calabresi 4, Kathleen Zackowski 1,2,4
1Motion Analysis Laboratory, Kennedy Krieger Institute, 2Physical Medicine & Rehabilitation, Johns Hopkins University School of Medicine, 3Johns Hopkins University School of Medicine, 4Department of Neurology, Johns Hopkins University School of Medicine

Hip weakness is a common symptom affecting walking ability in people with multiple sclerosis. Isolated muscle strengthening is a useful method to target specific weaknesses. This protocol describes a progressive resistance-training program using exercise bands to increase hip muscle strength.

image

Developmental Biology

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
Jason T. Schott *1, Leslie A. Kirby *1, Peter A. Calabresi 1, Emily G. Baxi 1
1Neurology, Johns Hopkins University, School of Medicine

Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.

image

Cancer Research

Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
Raffaella Spina 1, Dillon M. Voss 1, Laura Asnaghi 2, Andrew Sloan 1,3, Eli E. Bar 1
1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

An efficient screening protocol is presented for the identification of small molecules that promote astroglial differentiation in glioblastoma stem cells (GSCs). The assay is based on a stem cell differentiation reporter whereby the expression of the enhanced GFP (eGFP) is driven by the human GFAP promoter.

image

Neuroscience

Localization of the Locus Coeruleus in the Mouse Brain
Katharina Schmidt 1, Bilal Bari 2, Martina Ralle 3, Clorissa Washington-Hughes 1, Abigael Muchenditsi 1, Evan Maxey 4, Svetlana Lutsenko 1
1Department of Physiology, Johns Hopkins University, School of Medicine, 2Department of Neuroscience, Johns Hopkins University, 3Department of Molecular and Medical Genetics, OHSU, 4X-ray science division, Advanced Photon Source, Argonne National Laboratory

The locus coeruleus is a small cluster of neurons involved in a variety of physiological processes. Here, we describe a protocol to prepare mouse brain sections for studies of proteins and metals in this nucleus.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved