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University Paris Diderot, CNRS UMR 3571, Institut Pasteur

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
Laura Gouder 1,2,3, Jean-Yves Tinevez 4, Hany Goubran-Botros 1,2,3, Alexandra Benchoua 5, Thomas Bourgeron 1,2,3, Isabelle Cloëz-Tayarani 1,2,3
1Human Genetics and Cognitive Functions, Institut Pasteur, 2CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, 3Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, 5Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.

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Behavior

Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication
Allain-Thibeault Ferhat 1, Nicolas Torquet 2, Anne-Marie Le Sourd 1, Fabrice de Chaumont 3, Jean-Christophe Olivo-Marin 3, Philippe Faure 2, Thomas Bourgeron 1, Elodie Ey 1
1Human Genetics and Cognitive Functions, University Paris Diderot, CNRS UMR 3571, Institut Pasteur, 2Neurophysiology and Behavior, University Pierre et Marie Curie Paris 6, CNRS UMR 7102, 3Bio Image Analysis, CNRS URA 2582, Institut Pasteur

Mouse ultrasonic vocalizations are used as proxies to model the genetic bases of vocal communication deficits in mouse models for neuropsychiatric disorders. The present protocol describes three experimental contexts that reliably elicit ultrasonic vocalizations from pups (throughout development) and adult mice (same-sex interactions, male-estrus female interactions).

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