Carl Zeiss Microscopy GmbH

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Birgit Perner¹, Danny Schnerwitzki¹, Michael Graf^1,3, Christoph Englert^1,2

¹Molecular Genetics, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), ²Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, ³Carl Zeiss Microscopy GmbH

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

Developmental Biology

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Irene U. Wacker^1,2, Lisa Veith³, Waldemar Spomer^2,4, Andreas Hofmann^2,4, Marlene Thaler⁵, Stefan Hillmer⁶, Ulrich Gengenbach^2,4, Rasmus R. Schröder^1,2,3

¹Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, ²Heidelberg Karlsruhe Research Partnership (HEiKA), ³Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, ⁴Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), ⁵Carl Zeiss Microscopy GmbH, ⁶Electron Microscopy Core Facility, Universität Heidelberg

This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.