Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass SpectrometryEkaterina Boyarchuk 1, Philippe Robin 1, Lauriane Fritsch 1, Véronique Joliot 1, Slimane Ait-Si-Ali 1
1Epigenetics and Cell Fate, UMR 7216 CNRS, Centre National de la Recherche Scientifique CNRS - Université Paris Diderot, Sorbonne Paris Cité
MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.
