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University of Quebec in Montreal

1 ARTICLES PUBLISHED IN JoVE

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Genetics

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Adjimon Gatien Lokossou 1, Chirine Toufaily 2, Amandine Vargas 3, Benoit Barbeau 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.

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