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University Hospital of Zurich

4 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
Martin H. M. Sailer 1, Durga Sarvepalli 2, Catherine Brégère 3, Urs Fisch 3, Marin Guentchev 4, Michael Weller 6, Raphael Guzman 3, Bernhard Bettler 1, Arkasubhra Ghosh *2, Gregor Hutter *5
1Dept. of Biomedicine, Pharmacenter, University of Basel, 2Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, 3Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, 4Department of Neurosurgery, Klinikum Idar-Oberstein, 5Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 6Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich

Epithelial to mesenchymal transition (EMT) allows cancers to become invasive. To investigate EMT, a neural stem cell (NSC)-based in vitro model devoid of serum and enzymes is described. This standardized system allows quantitative and qualitative assessment of cell migration, gene and protein expression. The model is suited for drug discovery.

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Medicine

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure
Erik Schadde 1,2,3, Martin Hertl 2, Stefan Breitenstein 3, Beatrice Beck-Schimmer 1,4, Martin Schläpfer 1,4
1Institute of Physiology - Center for Integrative Human Physiology, University of Zurich, 2Department of Surgery, Rush University Medical Center, 3Department of Surgery, Cantonal Hospital Winterthur, 4Institute of Anesthesiology, University and University Hospital Zurich

Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. This model may elucidate mechanisms involved in rapid hypertrophy and allows testing of drugs that promote or block the acceleration of regeneration.

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Medicine

A Reproducible Intensive Care Unit-Oriented Endotoxin Model in Rats
Jan Heil 1, Martin Schläpfer 1,2
1Institute of Physiology, University of Zurich, 2Institute of Anesthesiology, University Hospital of Zurich

Here, we present a reproducible intensive care unit-oriented endotoxin model in rats.

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Neuroscience

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Jeffrey D. Messinger 1, Max Brinkmann 2, James A. Kimble 1, Andreas Berlin 1, K. Bailey Freund 3,4, Gregory H. Grossman 5, Thomas Ach *6, Christine A. Curcio *1
1Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham Heersink School of Medicine, 2Department of Ophthalmology, University Hospital of Zurich, 3Vitreous Retina Macula Consultants of New York, 4Department of Ophthalmology, New York University Grossman School of Medicine, 5Advancing Sight Network, 6Department of Ophthalmology, University Hospital Bonn

Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.

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