University Hospital of Zurich

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Martin H. M. Sailer¹, Durga Sarvepalli², Catherine Brégère³, Urs Fisch³, Marin Guentchev⁴, Michael Weller⁶, Raphael Guzman³, Bernhard Bettler¹, Arkasubhra Ghosh^*2, Gregor Hutter^*5

¹Dept. of Biomedicine, Pharmacenter, University of Basel, ²Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, ³Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, ⁴Department of Neurosurgery, Klinikum Idar-Oberstein, ⁵Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, ⁶Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich

Epithelial to mesenchymal transition (EMT) allows cancers to become invasive. To investigate EMT, a neural stem cell (NSC)-based in vitro model devoid of serum and enzymes is described. This standardized system allows quantitative and qualitative assessment of cell migration, gene and protein expression. The model is suited for drug discovery.

Medicine

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

Erik Schadde^1,2,3, Martin Hertl², Stefan Breitenstein³, Beatrice Beck-Schimmer^1,4, Martin Schläpfer^1,4

¹Institute of Physiology - Center for Integrative Human Physiology, University of Zurich, ²Department of Surgery, Rush University Medical Center, ³Department of Surgery, Cantonal Hospital Winterthur, ⁴Institute of Anesthesiology, University and University Hospital Zurich

Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. This model may elucidate mechanisms involved in rapid hypertrophy and allows testing of drugs that promote or block the acceleration of regeneration.

Medicine

A Reproducible Intensive Care Unit-Oriented Endotoxin Model in Rats

Jan Heil¹, Martin Schläpfer^1,2

¹Institute of Physiology, University of Zurich, ²Institute of Anesthesiology, University Hospital of Zurich

Here, we present a reproducible intensive care unit-oriented endotoxin model in rats.

Neuroscience

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Jeffrey D. Messinger¹, Max Brinkmann², James A. Kimble¹, Andreas Berlin¹, K. Bailey Freund^3,4, Gregory H. Grossman⁵, Thomas Ach^*6, Christine A. Curcio^*1

¹Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham Heersink School of Medicine, ²Department of Ophthalmology, University Hospital of Zurich, ³Vitreous Retina Macula Consultants of New York, ⁴Department of Ophthalmology, New York University Grossman School of Medicine, ⁵Advancing Sight Network, ⁶Department of Ophthalmology, University Hospital Bonn

Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.