University of Science and Technology, Daejeon, South Korea

1 ARTICLES PUBLISHED IN JoVE

Biology

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Haseong Kim¹, Kil Koang Kwon¹, Wonjae Seong¹, Seung-Goo Lee^1,2

¹Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, ²Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea

This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).