Nippon Dental University

3 ARTICLES PUBLISHED IN JoVE

Medicine

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Larry Kagemann^1,2, Bo Wang², Gadi Wollstein¹, Hiroshi Ishikawa^1,2, Brandon Mentley¹, Ian Sigal^1,2,3, Richard A Bilonick^1,4, Joel S Schuman^1,2,3

¹Department of Ophthalmology, UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, University of Pittsburgh School of Medicine, ²Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, ³The McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, ⁴Deptartment of Biostatistics, Graduate School of Public Health, University of Pittsburgh

Trabecular meshwork (TM) migration into Schlemm’s canal space can be induced by acute pressure elevation by ophthalmodynamometer, and observed by spectral domain optical coherence tomography. The goal of this method is to quantify the morphometric response of the living outflow tract to acute pressure elevation in living tissues in situ.

Medicine

A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side

Stefanie Dinner¹, Julia Borkowski¹, Carolin Stump-Guthier¹, Hiroshi Ishikawa², Tobias Tenenbaum¹, Horst Schroten¹, Christian Schwerk¹

¹Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University, ²Department of NDU Life Sciences, Nippon Dental University

The epithelial cells of the choroid plexus (CP) form the blood-cerebrospinal fluid barrier (BCSFB). An in vitro model of the BCSFB employs human choroid plexus papilloma (HIBCPP) cells. This article describes culturing and basolateral infection of HIBCPP cells using a cell culture filter insert system.

Education

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

Marianne Theile¹, Linus Wiora¹, Dominik Russ¹, Jonas Reuter¹, Hiroshi Ishikawa², Christian Schwerk³, Horst Schroten³, Stefan Mogk¹

¹Interfaculty Institute of Biochemistry, University of Tübingen, ²Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, ³Department of Pediatrics, Medical Faculty Mannheim, Heidelberg University

Here, we demonstrate how to set up an inexpensive volt-amperemeter with programmable output frequency that can be used with commercially available chopstick electrodes for transepithelial/endothelial electrical resistance measurements.