Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case StudyPaulina J. Stanczyk 1, F. Anthony Lai 1, Spyros Zissimopoulos 1
1Wales Heart Research Institute, Cardiff University School of Medicine, College of Biomedical and Life Sciences
Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca2+ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.
