Institute of Pathology, University of Bonn Medical School

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

Kristian Pajtler^*1, Anna Bohrer^*1, Jochen Maurer², Hubert Schorle², Alexander Schramm¹, Angelika Eggert¹, Johannes Hubertus Schulte¹

¹Department of Pediatric Oncology, University Children's Hospital Essen, ²Department of Developmental Pathology, Bonn Medical School, Institute of Pathology

To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.

Developmental Biology

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

Caroline Kubaczka¹, Hubert Schorle¹

¹Department of Developmental Pathology, Institute of Pathology, University of Bonn Medical School

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.