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Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

DOI :

10.3791/2380-v

November 27th, 2010

November 27th, 2010

12,424 Views

1Department of Pediatric Oncology, University Children's Hospital Essen, 2Department of Developmental Pathology, Bonn Medical School, Institute of Pathology

To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.

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