Fibroblast to Induced Trophoblast Stem Cell (iTSC) Conversion
5:10
Individual iTSC Line Isolation (Transdifferentiation Day 21-28)
7:02
Results: Representative Morphological Changes During MEF to iTSC Conversion
7:56
Conclusion
Transcript
The overall goal of this procedure is to generate bonafide induced trophoblast stem cells or iTSCs from murine fibroblasts. This method can help answer key questions in the stem cell field regarding the nature of the barrier separating the embryon
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Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.