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Hôpital Maisonneuve-Rosemont

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Preparation and Applications of Organotypic Thymic Slice Cultures
Aditi Sood *1,2, Mengqi Dong *1,2, Heather J. Melichar 1,3
1Centre de Recherche, Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology, Université de Montréal, 3Department of Medicine, Université de Montréal

We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.

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