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Medical Center-University of Freiburg

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury
Natalie Burkard 1, Wolfgang Baar 2,3, Sven Flemming 1, Nicolas Schlegel 1, Jakob Wollborn 2,3, Reinhard Schneider 4, Robert W Brock 5, Christian Wunder 6, Martin Alexander Schick 2,3
1Department of General, Visceral, Transplantation, Vascular and Paediatric Surgery, Department of Surgery I, University of Würzburg, 2Department of Anesthesiology and Critical Care, Medical Center-University of Freiburg, 3Faculty of Medicine, University of Freiburg, 4Department of Internal Medicine I, Division of Nephrology, University of Würzburg, 5Department of Physiology and Pharmacology, West Virginia University School of Medicine, Robert C. Byrd Health Science Center, 6Department of Anesthesiology and Intensive Care Medicine, Robert-Bosch-Krankenhaus

Acute kidney injury (AKI) is a severe complication in critically ill patients and is related with an increased mortality. Here, we present a reliable and reproducible in vivo model to mimic AKI under inflammatory conditions that might contribute towards understanding the pathogenesis of septic AKI.

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Medicine

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
Ramona A. Kopton 1,2,3, Cinthia Buchmann 1,2, Robin Moss 1,2, Peter Kohl 1,2, Rémi Peyronnet 1,2, Franziska Schneider-Warme 1,2
1Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen, Medical Center-University of Freiburg, 2Faculty of Medicine, University of Freiburg, 3Faculty of Biology, University of Freiburg

We present a protocol for evaluating the electromechanical effects of GtACR1 activation in rabbit cardiomyocytes. We provide detailed information on cell isolation, culturing and adenoviral transduction, and on functional experiments with the patch-clamp and carbon-fiber techniques.

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