Medical Center-University of Freiburg

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury

Natalie Burkard¹, Wolfgang Baar^2,3, Sven Flemming¹, Nicolas Schlegel¹, Jakob Wollborn^2,3, Reinhard Schneider⁴, Robert W Brock⁵, Christian Wunder⁶, Martin Alexander Schick^2,3

¹Department of General, Visceral, Transplantation, Vascular and Paediatric Surgery, Department of Surgery I, University of Würzburg, ²Department of Anesthesiology and Critical Care, Medical Center-University of Freiburg, ³Faculty of Medicine, University of Freiburg, ⁴Department of Internal Medicine I, Division of Nephrology, University of Würzburg, ⁵Department of Physiology and Pharmacology, West Virginia University School of Medicine, Robert C. Byrd Health Science Center, ⁶Department of Anesthesiology and Intensive Care Medicine, Robert-Bosch-Krankenhaus

Acute kidney injury (AKI) is a severe complication in critically ill patients and is related with an increased mortality. Here, we present a reliable and reproducible in vivo model to mimic AKI under inflammatory conditions that might contribute towards understanding the pathogenesis of septic AKI.

Medicine

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Ramona A. Kopton^1,2,3, Cinthia Buchmann^1,2, Robin Moss^1,2, Peter Kohl^1,2, Rémi Peyronnet^1,2, Franziska Schneider-Warme^1,2

¹Institute for Experimental Cardiovascular Medicine, University Heart Center Freiburg-Bad Krozingen, Medical Center-University of Freiburg, ²Faculty of Medicine, University of Freiburg, ³Faculty of Biology, University of Freiburg

We present a protocol for evaluating the electromechanical effects of GtACR1 activation in rabbit cardiomyocytes. We provide detailed information on cell isolation, culturing and adenoviral transduction, and on functional experiments with the patch-clamp and carbon-fiber techniques.