Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the ChickSusan Wilde 1, Malcolm P. Logan 1
1Randall Division of Cell and Molecular Biophysics, King's College London, Guy's Campus
Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.
