Eberhard Karls University of Tuebingen

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Yogesh Singh^1,2, Oliver A. Garden³, Florian Lang², Bradley S. Cobb¹

¹Department of Comparative Biomedical Sciences, The Royal Veterinary College, ²Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen, ³Department of Clinical Science and Services, The Royal Veterinary College

Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.