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Charité-Universitätsmedizin

6 ARTICLES PUBLISHED IN JoVE

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Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model
Odilo Engel 1, Sabine Kolodziej 1, Ulrich Dirnagl 1, Vincent Prinz 1
1Department for Experimental Neurology, Center for Stroke Research Berlin, Charité Universitätsmedizin

Filamentous occlusion of the Middle cerebral artery is a common model for studying ischemic stroke in mice.

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Education

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
Robert Szulcek 1, Harm Jan Bogaard 1, Geerten P. van Nieuw Amerongen 2
1Department of Pulmonary Diseases, Institute for Cardiovascular Research, VU University Medical Center, 2Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center

This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.

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Neuroscience

Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice
Simon H. Bayerl 1, Melina Nieminen-Kelhä 1, Thomas Broggini 2, Peter Vajkoczy 1, Vincent Prinz 1
1Department of Neurosurgery and Center for Stroke-research Berlin (CSB), Charité-Universitätsmedizin, 2Department of Physics, University of California San Diego

Surgical occlusion of a distal middle cerebral artery branch (MCAo) is a frequently used model in experimental stroke research. This manuscript describes the basic technique of permanent MCAo, combined with the insertion of a lateral cranial window, which offers the opportunity for longitudinal intravital microscopy in mice.

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Immunology and Infection

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
Xue D. Manz 1, Hugo J. Albers 2,3, Petr Symersky 4, Jurjan Aman 1, Andries D. van der Meer 3, Harm Jan Bogaard 1, Robert Szulcek 1,5,6
1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, 5Institute of Physiology, Charité-Universitätsmedizin, 6German Heart Center

We present an in vitro vascular disease model to investigate whole blood interactions with patient-derived endothelium. This system allows the study of thrombogenic properties of primary endothelial cells under various circumstances. The method is especially suited to evaluate in situ thrombogenicity and anticoagulation therapy during different phases of coagulation.

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Medicine

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry
Wen-Jia Liu *1,2, Lisa Ernst *2, Benedict Doorschodt 2, Jan Bednarsch 1, Felix Becker 3, Richi Nakatake 2, Yuki Masano 2, Ulf Peter Neumann 1,4, Sven Arke Lang 1, Peter Boor 5, Isabella Lurje 6, Georg Lurje 1,6, René Tolba 2, Zoltan Czigany 1,2
1Department of Surgery and Transplantation, Faculty of Medicine, University Hospital RWTH Aachen, 2Institute for Laboratory Animal Science, Faculty of Medicine, University Hospital RWTH Aachen, 3Department of General-, Visceral-, and Transplantation Surgery, University Hospital Muenster, 4Department of Surgery, Maastricht University Medical Centre (MUMC), 5Institute of Pathology, Faculty of Medicine, University Hospital RWTH Aachen, 6Department of Surgery, Campus Charité Mitte | Campus Virchow-Klinikum, Charité-Universitätsmedizin

Large animal models play an essential role in preclinical transplantation research. Due to its similarities to the clinical setup, the porcine model of orthotopic kidney auto-transplantation described in this article provides an excellent in vivo setting for the testing of organ preservation techniques and therapeutic interventions.

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Neuroscience

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)
Shuheng Liu *1, Katharina Tielking *1, Dario von Wedel 1, Melina Nieminen-Kelhä 1, Susanne Mueller 2,3, Philipp Boehm-Sturm 2,3, Peter Vajkoczy 1, Ran Xu 1
1Department of Neurosurgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Department of Experimental Neurology and Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3NeuroCure Cluster of Excellence and Charité Core Facility 7T Experimental MRIs, Charité - Universitätsmedizin Berlin

Here we present a standardized SAH mouse model, induced by endovascular filament perforation, combined with magnetic resonance imaging (MRI) 24 h after operation to ensure the correct bleeding site and exclude other relevant intracranial pathologies.

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