CHU de Nantes

3 ARTICLES PUBLISHED IN JoVE

Medicine

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

Audrey Ayer¹, Frederic Borel¹, Francois Moreau¹, Xavier Prieur¹, Michel Neunlist², Bertrand Cariou³, Claire Blanchard^*1,4, Cedric Le May^*1

¹l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, ²CHU Nantes, Institut des Maladies de l'Appareil Digestif, INSERM U913, ³l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, CHU Nantes, ⁴Service de Clinique Chirurgicale Digestive et Endocrinienne, CHU de Nantes

Bariatric surgery is the most efficient way to reduce body weight and the deadly metabolic complications (diabetes, obesity, and dyslipidemia) frequently associated with morbid obesity. Mouse models of bariatric surgery represent a unique asset for deciphering molecular mechanisms behind the beneficial effects of these surgeries on diabetes, hypertension, and dyslipidemia.

Medicine

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

Samuel Frey^1,2, Audrey Ayer¹, Thibaud Sotin¹, Victoria Lorant¹, Bertrand Cariou¹, Claire Blanchard^*1,2, Cédric Le May^*1

¹CHU Nantes, CNRS, INSERM, l'institut du thorax, Nantes Université, ²Chirurgie Cancérologique, Digestive et Endocrinienne, Institut des Maladies de l'Appareil Digestif, Hôtel Dieu, CHU Nantes

Single-anastomosis duodeno-ileal bypass (SADI-S) is an emerging bariatric procedure with important metabolic effects. In this article, we present a reliable and reproducible model of SADI-S in mice.

Medicine

Developing a Clinically Relevant Hemorrhagic Shock Model in Rats

Thomas Dupas^*1, Virginie Aillerie^*1, Amandine Vergnaud¹, Thomas Pelé¹, Antoine Persello¹, Angélique Blangy-Letheule¹, Angélique Erraud¹, Mortéza Erfanian¹, Agnès Hivonnait¹, Anaïs Maillard¹, Jules Lecomte², Edith Bigot-Corbel³, Aurélia A. Leroux^1,4, Manon Denis², Bertrand Rozec², Benjamin Lauzier¹

¹CNRS, INSERM, l’institut du thorax, Nantes Université, ²CNRS, INSERM, l’institut du thorax, Nantes Université, CHU Nantes, ³Departement of Biochemistry, CHU de Nantes, ⁴Oniris

Hemorrhagic shock kills 1.9 million people worldwide every year. Small animals are frequently used as hemorrhagic shock models but are associated with issues of standardization, reproducibility, and clinical significance, thus limiting their relevance. This article describes developing a new clinically relevant hemorrhagic shock model in rats.