Institute of Science and Technology Austria

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

¹Membrane Biology Group, Centre for integrative Physiology, University of Edinburgh

A live fluorescence imaging technique to quantify the replenishment and mobilisation of specific synaptic vesicle (SV) pools in central nerve terminals is described. Two rounds of SV recycling are monitored in the same nerve terminals providing an internal control.

Biology

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Daniel von Wangenheim¹, Robert Hauschild², Jiří Friml¹

¹Developmental and Cell Biology of Plants, Institute of Science and Technology Austria, ²Bioimaging Facility, Institute of Science and Technology Austria

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

Neuroscience

A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

Javier Masis^1,2, David Mankus², Steffen B.E. Wolff^2,3, Grigori Guitchounts^1,2, Maximilian Joesch⁴, David D. Cox^1,2

¹Department of Molecular and Cellular Biology, Harvard University, ²Center for Brain Science, Harvard University, ³Department of Organismic and Evolutionary Biology, Harvard University, ⁴Institute of Science and Technology Austria

This article describes a straightforward method to prepare small animal brains for micro-CT imaging, in which lesions can be quantified and electrodes located with high precision in the context of the whole brain.

Biology

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Robert Beattie¹, Carmen Streicher¹, Nicole Amberg¹, Giselle Cheung¹, Ximena Contreras¹, Andi H. Hansen¹, Simon Hippenmeyer¹

¹Institute of Science and Technology Austria

A protocol to perform lineage tracing and functional genetic analysis of candidate genes at a single cell level using mosaic analysis with double markers (MADM) is presented. MADM clonal analysis provides a quantitative framework to measure the proliferative behavior, cellular output, and lineage relationship of individual progenitors and their daughter cells.