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CNRS, UPMC, UMR 7622, INSERM ERL U1156

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
Estelle Hirsinger 1, João Emanuel Carvalho 2, Christine Chevalier 1,3, Georges Lutfalla 4, Jean-François Nicolas 1, Nadine Peyriéras 5, Michael Schubert 2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

We report here the robust and efficient expression of fluorescent proteins after mRNA injection into unfertilized oocytes of Branchiostoma lanceolatum. The development of the microinjection technique in this basal chordate will pave the way for far-reaching technical innovations in this emerging model system, including in vivo imaging and gene-specific manipulations.

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Developmental Biology

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
Estelle Hirsinger 1,3, Ben Steventon 1,2
1Department of Developmental and Stem Cell Biology, Institut Pasteur, 2Department of Genetics, University of Cambridge, 3IBPS- Laboratoire de Biologie du Developpement (LBD), CNRS, UPMC, UMR 7622, INSERM ERL U1156

Here, we present a versatile mounting method that allows for the long-term time-lapse imaging of the posterior body development of live zebrafish embryos without perturbing normal development.

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