Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron TomographySylvain Trépout 1, Philippe Bastin 2, Sergio Marco 1
1Institut Curie, INSERM U1196, Campus Universitaire d'Orsay, 2Institut Pasteur, Trypanosome Cell Biology Unit, Department of Parasites & Insect Vectors, INSERM U1201
This report describes a sample preparation protocol and specific imaging conditions for performing scanning transmission electron tomography of thick biological specimens.