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St. John Provindence - Providence Park Hospital

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
Naimisha Beeravolu 1,2, Christina McKee 1,2, Ali Alamri 1,2, Sasha Mikhael 3, Christina Brown 1,2, Mick Perez-Cruet 2,4, G. Rasul Chaudhry 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System

Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.

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